Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves

doi:10.3969/j.issn.2095-4344.2014.34.017

Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (34): 5509-5514.doi: 10.3969/j.issn.2095-4344.2014.34.017

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Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves

Zhang Ben^{1, 2}, Gong De-jun¹, Zhang Xi-wu¹, Xu Tong-yi¹, Han Lin¹, Tang Hao¹, Lu Fang-lin¹, Xu Zhi-yun¹

¹Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; ²Centre of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510010, Guangdong Province, China

Revised:2014-06-08 Online:2014-08-20 Published:2014-08-20
Contact: Xu Zhi-yun, Professor, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
About author:Zhang Ben, Studying for doctorate, Attending physician, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; Centre of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Region, Guangzhou 510010, Guangdong Province, China Gong De-jun, Technician in charge, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China Zhang Ben and Gong De-jun contributed equally to this work.
Supported by:
the Military Medical Research during the Twelfth Five-Year Plan, No. BWS11C008

Abstract

Abstract:

BACKGROUND: Our preliminary study found that the monocusp valves made of ultramicropore expanded polytetrafluoroethylene (ePTFE) revealed no significant thrombus, calcification, or degradation 20 weeks after implanted into the descending aorta and the left pulmonary artery in sheep, which verified the good property of ePTFE. However, the surface of ePTFE in the left pulmonary artery was covered with obvious neointima.

OBJECTIVE: To assess the biocompatibility of phosphorylcholine-coated ePTFE.

METHODS: ePTFE surface was modified by phosphorylcholine derivative. Then the changes of surface shape, tensile stress at yield and elasticity modulus, water contact angle, and protein absorption capacity of ePTFE after surface modification were observed. (1) Hemolytic test: the leaching solution of phosphorylcholine-coated ePTFE, leaching solution of uncoated ePTFE, normal saline, and distilled water were added to the diluted human blood, respectively. (2) Platelet count test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, high density polyethylene, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively. (3) Platelet activation test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, γ-Globulins, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively.

RESULTS AND CONCLUSION: The mean micropore diameter of ePTFE was significantly decreased after phosphorylcholine coating (P < 0.001). The hydrophilicity and the ability of suppressing protein adsorption were significantly strengthened after phosphorylcholine coating (P < 0.001). Phosphorylcholine coating did not influence ePTFE in biomechanical properties and hemolytic test. The platelet count test and platelet activation test demonstrated that phosphorylcholine coating significantly improved anti-thrombus function of ePTFE. So, phosphorylcholine coating can enhance anti-thrombus function, suppress protein adsorption, and improve biocompatibility of ePTFE.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: polytetrafluoroethylene, phosphorylcholine, materials testing

CLC Number:

R318

Zhang Ben, Gong De-jun, Zhang Xi-wu, Xu Tong-yi, Han Lin, Tang Hao, Lu Fang-lin, Xu Zhi-yun. Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(34): 5509-5514.

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References

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Phosphorylcholine coating enhances biocompatibility of expanded polytetrafluoroethylene used in polymeric prosthetic heart valves

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